Biofuel and bioenergy produced from biowastes and biomass is a clean energy source which can be produced renewably. The 21 chapters of this book provide state-of-the-art reviews, current research, and technology developments with respect to 1st, 2nd, and 3rd generation biofuels and bioenergy. The book focuses on the biological/biochemical pathway, as this option has been reported to be the most cost-effective method for biofuel/bioenergy production. The opening chapter covers the overview of the current status of biofuel and bioenergy production. The rest of the chapters are grouped into seven categories; they cover biomethane production, microbial fuel cells, feedstock production, preprocessing, biomass pretreatment, enzyme hydrolysis, and syngas fermentation. Algal processes for biofuel production, biobutanol production, bioreactor systems, and value-added processing of biofuel residues are included. This book addresses life cycle analyses (LCA) of 1st and 2nd generation biofuels (from corn, soybean, jatropha, and cellulosic biomass) and the emerging applications of nanotechnology in biofuel/bioenergy production. The book is organized in such a way that each preceding chapter builds a foundation for the following one. At the end of each chapter, current research trends and further research needs are outlined. This is one of the first books in this emerging field of biofuel/bioenergy that provides in-depth technical information on the broad topics of biofuel and bioenergy with extensive illustrations, case studies, summary tables, and up-to-date references. This book will be valuable to researchers, instructors, senior undergraduate and graduate students, decision-makers, professionals, and others interested in the field of biofuel/bioenergy.